MFI Bot1 Autosampler is also available, and can be added anytime to automate the whole process. Ella . https://doi.org/10.1002/elps.200410075, Siedler M (2011) Implementation of a platform approach for early biologics development. Besides ProteinSimple, Bio-Technes brands also include Tocrus, Novus Biologicals, and R&D Systems.. To learn more, visit Bio-Technes website. Minneapolis, MN 55413 Electrophoresis 26:556562. SimpleSol Protein Solubilizer, 200 mL. FAX 612 656 4400. PubMed ProteinSimple recently merged with Bio-Techne, and is now a part of the companys Protein Platforms division. Micro-flow Imaging (MFITM) technology provides more insights into sub-visible particles like particle population differentiating and classification. Toll-free 800 343 7475 Fisher Scientific is always working to improve our content for you. All contact information provided shall also be maintained in accordance with our ProteinSimple. ProteinSimple branded instruments are available to quote. ACD branded products will be available on biotechne.com in the near future. J Chromatogr B Biomed Appl 714:113118. Imaged capillary isoelectric focusing (icIEF) is a gold standard method for characterizing the charge heterogeneity of protein therapeutics. In regard to the volume of applicable uses of their products, they are immense. The on-board sample mixing feature further enhances the ease-of-use, and reduces operator-dependent variability commonly encountered with other systems. Novus Biologicals branded products are not currently available on biotechne.com and can be found at novusbio.com. Presented at CE in the Biotechnology & Pharmaceutical Industries: symposium on the practical applications for the analysis of proteins, nucleotides and small molecules, Monday, Sept 30, 2019, Bethesda, MD, USA. https://doi.org/10.1007/978-1-0716-1186-9_8, DOI: https://doi.org/10.1007/978-1-0716-1186-9_8, Over 10 million scientific documents at your fingertips, Not logged in J Sep Sci 35:31243129. Platform methods, which can be applied without modification to the analysis of different protein therapeutics, save valuable time and resources in method development and quality control. MAbs 4:578585.
A platform method for charge heterogeneity - ScienceDirect Part of Springer Nature. iCE systems are fluent in capillary isoelectric focusing (cIEF) and capillary electrophoresis sodium dodecyl sulfate (CE-SDS). ProteinSimple branded instruments are available to quote. Automated Capillary Electrophoresis: A Technological Renaissance at the Heart of Biopharmaceuticals, Joan Kicks Her Method Development into High Gear with iCE3, View All Products Compatible with Maurice, Download 046-296 RevD Maurice cIEF Method Development Guide web, Download 046-297 Maurice CE-SDS Application Guide revG, Reduced IgG: 25 min, Non-reduced IgG: 35 min, 100 guaranteed, 200 maximum for CE-SDS, 500 maximum for CE-SDS PLUS, 0.05 pI units (for wide range 310 ampholytes), 0.7 g/mL (Native fluorescence) 3.0 g / mL(Absorbance) (Values based on a monoclonal antibody), 0.3 g/mL (Value based on Internal Standard), Maurice - CE-SDS & icIEF iCE Platform |ProteinSimple. "I think that the data we produced [on Maurice] shows excellent quality, due to the precise and reliable Maurice instrument. ProteinSimple SimpleSol Protein Solubilizer for cIEF, 200mL. For now, you can find them on their website. SimpleSol Protein Solubilizer for cIEF (046-575). Using these systems, heterogeneity in Cancer and Immuno-oncology samples can be studied. PubMed Copyright 2023 Bio-Techne. The protein tools they have developed hold various applications like protein and antibody characterization, bioprocessing, protein quantitation, stability, and formulation testing in the medical, biotech, life science, and pharmaceutical industries. Toll-free 800 343 7475 This technical note introduces SimpleSol, a novel and alternative reagent to urea to solubilize proteins for icIEF analysis on iCE and Maurice systems. Product Documents. ", Professor Hermann Wtzig, The Simple Plex range includes ELA and Simple Plex Assays that facilitate laboratory automation. Nucleoproteins: These proteins are composed of simple . The fast separation time, flexible workflow and high reproducibility makes Maurice a true lab workhorse. In addition, when dealing with molecules such as ADCs, no more hassle about handling the toxic waste. PubMedGoogle Scholar. To uncover the role of proteins in cancer and accelerate the analysis of protein-based therapeutics, ProteinSimples Advanced Western Blotting solutions make the study of proteins, their function, and signaling mechanisms easier. ProteinSimple offers the worlds first automated Single-Cell Western platform: MiloTM. ", Joan Garrison, In the absence of a stabilizer, Sample 1 aggregated during analysis by IEF, creating a non-reproducible peak pattern (Fig. Technical University of Braunschweig in Germany, "The short run times and easy sample preparation allowed for method development within a day or two. Western Protein Analysis problem solvers include Jess, Abby, Wes, Sally Sue, Peggy Sue, and NanoPro 1000. Here, we provide platform methods for icIEF analysis of three classes of protein therapeutics, a biosimilar to the monoclonal antibody trastuzumab, recombinant human erythropoietin (rhEPO), and a fusion protein. The versatility of the instrument allows the possibility of offering clients rapid alternatives to traditional ID and charge heterogeneity assays. This technical note introduces SimpleSol, a novel and alternative reagent to urea to solubilize proteins for icIEF analysis on iCE and Maurice systems. Imaged capillary isoelectric focusing (icIEF) is a gold standard method for characterizing the charge heterogeneity of protein therapeutics. In all tests, more than three injections were performed from a single sample preparation under each condition to observe the reproducibility. Simple Western systems have the ability to separate proteins by size or charge and help develop and characterize vaccine candidates. To access this literature content please fill out the form below. No laborious capillary assembly or maintenance is required. https://doi.org/10.1002/jssc.201200633, CrossRef Using TcBusterTM Technology you can carry out genome engineering with non-viral transgene insertion. CAS With fully automated Simple Westerns you can quantify Degrader induced protein knockdown even with low sample volume. This helps get precise, quantitative data for accurate determination of DC50 curve values. Setting the standard in quality research reagents for over 30 years, A trusted leader in quality life science reagents, Your trusted supplier for innovative and high performance life science reagents, Proprietary systems and consumables for simpler, more quantitative and affordable protein analysis, Proprietary RNAscope technology capable of detecting and quantifying RNA biomarkers in situ at single molecule sensitivity, A world leader in developing liquid biopsy based diagnostics, 614 McKinley Place NE ProteinSimple also conducts webinars and training sessions to help consumers learn more about their products. In one day you can complete your method development, or analyze 48 to 100 samples in one batch. See terms and conditions for offer details. Lease the cutting-edge lab equipment you need, save capital for other parts of your business, and achieve R&D milestones quicker. Waste is collected inside the cartridge which removes the concern about cross-contamination between experiments. - 173.212.237.43. And because proteins tend to aggregate less at lower concentrations you can reduce or even eliminate urea completely in some of your methods. In one day you can complete your method development, or analyze 48 to 100 samples in one batch. Consider leasing through Excedr to save your lab time and money. Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments. For Vaccine development processes, ProteinSimple provides advanced western blotting solutions. Dont have the budget to purchase lab equipment outright? All Rights Reserved. USA, TEL 612 379 2956 The fast separation time, flexible workflow and high reproducibility makes Maurice a true lab workhorse.
Chris Heger, Ph.D. - Director, Applications Science - LinkedIn Simple Western Systems help perform serum antibody analysis, and multi-analyte characterization of Humoral response. ProteinSimples portfolio of protein tools includes a wide range of products, from simple western immunoassay systems that quantify protein expression to biological systems that probe the structure and purity of protein-based therapeutics. By continuing to use this website you acknowledge this and agree to our cookie policy. Using dTag degradation technology, you can target and degrade any intracellular protein of interest. With a comprehensive range of analytical instruments, ProteinSimple provides its customers an easier, faster, and more affordable way to conduct protein analysis. ProteinSimple branded instruments are available to quote.
Simple protein Definition & Meaning | Dictionary.com Correspondence to Custom Targeted Protein Degradation Services, Certificate of Analysis (COA) Finder Tool. For safe and effective treatments using cell and gene therapies, ProteinSimple has a range of next-generation analytical solutions to offer. Not finding your part number? Minneapolis, MN 55413 USA, TEL 612 379 2956 https://doi.org/10.1002/elps.201800118, Zhang X, Voronov S, Mussa N, Li Z (2017) A novel reagent significantly improved assay robustness in imaged capillary isoelectric focusing. A hands-free, easy to use system that provides highly precise and accurate biomarker detection. 1).In the presence of 2 M urea, the peak pattern of Sample 1 became more reproducible (Fig. PubMed Central ProteinSimples products and solutions will soon be available on Bio-Technes website to enable consumers to access the brands products and services in one place. $135.22 / Each of 1. ProteinSimple is known for manufacturing and supplying analytical instrumentation and consumables for protein research. The icing? This technology is fluent in cIEF and CE-SDS, makes it easy to operate, is a time saver, and is dependable. FluorChem Systems perform western blot imaging. Bio-Rad Laboratories GmbH, Feldkirchen, Germany, 2021 Springer Science+Business Media, LLC, part of Springer Nature, Wu, J., McElroy, W., Haitjema, C., Lck, C., Heger, C. (2021). For use with Maurice, Maurice C. and iCE3 systems. These are easy, high-performing and budget-friendly. ProteinSimple is a leader in exosome isolation and also provides high-sensitivity western blotting solutions. Eastern North America. Request your COA here. Maurice produces pI and charge heterogeneity data in less than 10 minutes, and size-based CE-SDS data in 35 minutes. The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. However, we are committed to improving your shopping experience. Google Scholar, Salas-Solano O, Kennel B, Park SS et al (2012) Robustness of iCIEF methodology for the analysis of monoclonal antibodies: an interlaboratory study. Setting the standard in quality research reagents for over 30 years, A trusted leader in quality life science reagents, Your trusted supplier for innovative and high performance life science reagents, Proprietary systems and consumables for simpler, more quantitative and affordable protein analysis, Proprietary RNAscope technology capable of detecting and quantifying RNA biomarkers in situ at single molecule sensitivity, A world leader in developing liquid biopsy based diagnostics, 614 McKinley Place NE Maurice's native fluorescence detection for cIEF provides 3-5X higher sensitivity than UV absorption, which means you can now measure sample at concentration as low as 0.7 g/ml. Headquartered in Orchard Parkway, San Jose, California, ProteinSimple is helping scientists discover answers to real-world problems through protein and disease research. ProteinSimple is known for manufacturing and supplying analytical instrumentation and consumables for protein research. ProteinSimple, now a brand of Bio-Techne, is transforming protein research with their analytical protein instruments for easy analysis and rethinking protein tools. As the name suggests, the company aims to simplify protein research and help thousands of researchers around the world with their diverse portfolio of protein tools. For use with Maurice, Maurice C. and iCE3 systems. Custom Targeted Protein Degradation Services, Certificate of Analysis (COA) Finder Tool. MAbs 11:11131121. Please sign in to view account pricing and product availability.
QC Method Transfer Scientist, Cook Pharmica. Maurice employs a prequalified, ready-to-use cartridge design which fully automates column conditioning and clean up. It is essential in conducting research in biosimilar characterization, cell and gene therapy, and protein aggregation and particle analysis. Browse your leasing options today! Their products are being used in fields like neuroscience, oncology, bioinformatics, RNA research, cell and gene therapy, and more. In: Posch, A. Electrophoresis 39:20912098. You can develop platform methods and use them for multiple molecules too. Headquartered in Orchard Parkway, San Jose, California, ProteinSimple is helping scientists discover answers to real-world problems through protein and disease research. Bio-Techne uses cookies to provide you with a great website experience. By continuing to use this website you acknowledge this and agree to our cookie policy. Learn more, Hjertn S, de Zhu M (1985) Adaptation of the equipment for high-performance electrophoresis to isoelectric focusing. R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through biotechne.com. Platform Methods to Characterize the Charge Heterogeneity of Three Common Protein Therapeutics by Imaged Capillary Isoelectric Focusing. This is a preview of subscription content, access via your institution. Store the unopened product at 2 - 8 C. By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. CAS Qty Check Availability. Catalog No. We also lease a wide variety of lab equipment, from general lab, clinical, and analytical instruments to spectroscopy and biotech equipment. Novus Biologicals branded products are not currently available on biotechne.com and can be found at novusbio.com. Imaged capillary isoelectric focusing (icIEF), https://doi.org/10.1007/978-1-0716-1186-9_8, https://doi.org/10.1016/S0021-9673(00)90512-0, https://doi.org/10.1016/S0378-4347(98)00036-X, https://doi.org/10.1080/19420862.2019.1628589, https://submissions2.mirasmart.com/Verify/CEPharm2019/submission/temp/rad3B853.pdf, https://www.proteinsimple.com/literature_download.html?docid=1817, Tax calculation will be finalised during checkout.
SimpleSol Protein Solubilizer for cIEF (046-575) | Bio-Techne
Hill Country Yarn Crawl 2022,
Philips Respironics System One Specifications,
Thycotic Session Recording,
John Deere Lt160 Deck,
Articles P