Int. Moreover, A. muciniphila may have a value in modifying cancer treatment. Sci. CAS Pasteurized Akkermansia muciniphila increases whole-body energy expenditure and fecal energy excretion in diet-induced obese mice. Moreover in recent years, the propensity towards using non-viable bacterial strains as alternative products to prevent the potential risks of probiotics has increased, especially in high-risk individuals such as infants, the elderly and immunocompromised patients30. As an abundant resident in the intestinal tract of humans and animals, the probiotic effects of A. muciniphila including metabolic modulation, immune regulation and gut health protection, have been widely investigated. 81, 67496756 (2015). Ashrafian, F. & Behrouzi, A. Akkermansia muciniphila is a promising probiotic - Zhang - 2019 Google Scholar. 001 were considered statistically significant, respectively. 72, 1829 (2014). Gene expression analysis showed tight junction proteins were upregulated, similar to mice's colon. 73, 2437 (2019). Google Scholar. & Corr, S. C. Establishing boundaries: The relationship that exists between intestinal epithelial cells and gut-dwelling bacteria. Food Sci. Collado, M. C. et al. A critical review of the relationship between dietary components, the gut microbe Akkermansia muciniphila, and human health. J. Neuroinflammation 15, 11 (2018). Pendulum Akkermansia | Akkermansia Pendulum UK | Turmeric & Honey broad-spectrum probiotic clinically shown to . In our study, we demonstrated lowering-body, -liver, and -eWAT weight impact of both forms of A. muciniphila in study groups. Endocrinol. A review of a potential and promising probiotic candidateAkkermansia 18, 112 (2019). Zhai, Q., Feng, S., Arjan, N. & Chen, W. A next generation probiotic, Akkermansia muciniphila. Try Glucose Control CAS Moreover, biochemical parameters and inflammatory biomarkers in plasma demonstrated that pasteurized A. muciniphila had more pronounce effect. After 21days of culture, Caco-2 monolayer was exposed to a live and pasteurized A. muciniphila at Multiplicity of infection (MOI) ratio of 100 (bacteria per cell) as well as an equal volume of PBS was used as a control for bacterial treatment. Then, histological slides were evaluated using a light microscope (Olympus SX-21) equipped with a digital Dino-Lite lens and Dino-capture 2 software (AnMo Electronics Corp., New Taipei City, Taiwan)26. Microbiol. 01, and P<0. ppar-, ppar-, and tgf-) in the liver of normal diet-fed mice. The latter are connections (membrane proteins) between the cells of the intestinal mucosa and ensure that important nutrients are selected and separated from harmful substances. (UK) Limited is an appointed representative of eBay Commerce UK Ltd (of Hotham House, 1 Heron Sq, Richmond upon Thames, Greater London, TW9 . https://doi.org/10.1128/AEM.01477-07 (2007). CAS Rev. Rev. Sanders, M. E. et al. This category only includes cookies that ensures basic functionalities and security features of the website. 25, 10961103 (2019). In comparison with viable probiotics, para-probiotics are non-viable and have more effective properties9,17. A comparative study of the antidiabetic effects exerted by live and dead multi-strain probiotics in the type 2 diabetes model of mice. Stained sections were evaluated by an expert pathologist, blind to study groups. Akkermansia muciniphila as a Next-Generation Probiotic in Modulating Pathol. RNeasy Plus Mini kit (Qiagen, USA, Cat No. & Kim, J. Appreciation of the importance of Akkermansia muciniphila is growing, and it is becoming increasingly relevant to identify preventive and/or therapeutic solutions targeting gut-liver-brain axes for multiple diseases via Akkermansia muciniphila. They cleared in xylene and impregnated and embedded in paraffin. & Im, S.-H. Probiotics as an immune modulator. Microbiol. Mol. Probiotic Bifidobacterium lactis V9 attenuates hepatic steatosis and inflammation in rats with non-alcoholic fatty liver disease. Akkermansia Muciniphila: The Most Powerful Probiotic You've - BodyBio Probiotics for Gut Health and IBS Relief. Microbiol. Borgeraas, H., Johnson, L., Skattebu, J., Hertel, J. Hepatol. # RR037A), and 2X SYBR Premix Ex Taq II (Tli RNase H, Plus Takara, Japan, Cat. (A) Histopathology of colon (Black arrows: crypt depth, and black arrowheads: mucous thickness) (4 samples per group). Rep. 8, 115 (2018). J. Diabetes Metab. Higher level of anti-inflammatory cytokine (IL-10) and lower levels of the pro-inflammatory cytokine (TNF- and IL-6) were found in the plasma of Am and PAm group, respectively. CAS MegaSporeBiotic boasts a 5-year shelf-life, does not require refrigeration, and maintains efficacy during antibiotic therapy. Akkermansia muciniphila (A. muciniphila), an intestinal symbiont colonizing in the mucosal layer, is considered to be a promising candidate as probiotics.A. Microbiome Labs UK - UK distributors of MegasporeBiotic, RestorFlora, HU58, MegaQuinone K2-7 and the rest of the Microbiome Labs product range. J. Diabetes Metab. Regarding, recent investigations exhibited that probiotics improved to maintain balance immunity and inflammation-related cytokines in healthy mice46 and subjects47. In the liver, the hepatocytes and sinusoidal liver cells in Am and PAm groups were similar to control group. 60, 22652276 (2020). Google Scholar. tlr-4, tnf-, and il-10) and (C) lipid metabolism-related genes (e.g. Adipose tissue is an immunological organ that plays a key role in the homeostasis of energy and stores extra lipids61. level (p value 0.04) (Fig. (A) Histopathology of liver (White Arrows: accumulation of lipids droplets) (4 samples per group). Inform. https://doi.org/10.1136/gutjnl-2014-308778 (2016). 31, 545561 (2015). Our research provided an insight into the beneficial effects of A. muciniphila on host health and the safety of its use in normal diet-fed mice. non-digestible oligosaccharides that can increase microbial diversity and selectively feed beneficial bacteria like Akkermansia muciniphila, . PubMed *p<0.05. We found that orally administrated both forms of A. muciniphila triggered hepatic immune response-/lipid metabolism-related genes in study groups, which indicated improving liver health. Effects of probiotics on gut microbiota: Mechanisms of intestinal immunomodulation and neuromodulation. S.D., S.K., M.D.O., F.V., and A.M. revised the manuscript. In addition, the regulation of lipid metabolism by up-regulating angptl4 was observed after both treatments, whereas Am group was more noticeable (p value 0.0005) (Fig. performed the most of experiments, analyzed data and wrote the manuscript. Both treatments increased il-10 expression, while the live form had a greater effect on maintain liver health (p value<0.0001) (Fig. Cani, P. D. & de Vos, W. M. Next-generation beneficial microbes: The case of Akkermansia muciniphila. Plovier, H. et al. Nat. You also have the option to opt-out of these cookies. The authors declare no competing interests. Intestinal integrity and Akkermansia muciniphila, a mucin-degrading member of the intestinal microbiota present in infants, adults, and the elderly. Moreover, the tissue specimens are saved for histological staining. Akkermansia muciniphila: from its critical role in human health to Marcellin, P. & Kutala, B. K. Liver diseases: A major, neglected global public health problem requiring urgent actions and large-scale screening. Akkermansia Muciniphila: A Simple Overview (2020) - Healthpath Out of these, the cookies that are categorized as necessary are stored on your browser as they are essential for the working of basic functionalities of the website. 20, 16 (2021). 33 results for "akkermansia muciniphila" RESULTS Pure Encapsulations - Poly-Prebiotic Powder - Targets Akkermansia Muciniphila to Support GI Barrier Function - 4.9 Ounces 4.3 (55) 8672 (624.28/kg)89.72 Get it Monday, 6 Mar - Wednesday, 8 Mar FREE Delivery Akkermansia Muciniphila Probiotic Granules * Live Long * Weight Control Dogadan Bizim (5) In these regards, A. muciniphila, which grown in mucin-based medium, induced an increase in intestinal integrity and modulated immune response in ND mice and cell line18,20,51. CAS 73, 77677770. Article M Derrien EE Vaughan CM Plugge WM Vos de 2004 Akkermansia muciniphila gen nov, sp nov, a human intestinal mucin-degrading bacterium. For this reason, therapies that only address one of these facets do not typically provide total restoration of a dysfunctional GI tract. Rep. 10, 112 (2020). muciniphila is known to have an important value in improving the host metabolic functions and immune responses. *, **, *** 'P<0.05, P<0. Therefore, we assessed the effects of alive and pasteurized A. muciniphila on morphology and intestinal barrier-related genes in the colon of ND-fed mice. Med. Crit. Amazon.co.uk: Akkermansia Muciniphila BMC Med. Front. Cross-talk between Akkermansia muciniphila and intestinal epithelium controls diet-induced obesity. Taken together, these observations in our and other experiments have shown that the administration of A. muciniphila provided some health benefits in multiple tissues, which are correlated with lowering the risk of various diseases. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate. Food Sci. Appl. PubMed A non-dairy probiotics (poi) influence on changing the gastrointestinal tracts microflora environment. Differences between groups were calculated using one-way analysis of variance (ANOVA) followed by Tukeys post hoc test for multiple comparisons between more than two groups. A.B. A. muciniphila MucT (ATCC BAA-835) was cultured in a synthetic medium under the anaerobic conditions as previously described17. The results showed that alive A. muciniphila significantly reduced the abundance of Firmicutes (p value 0.02) and increased Bacteroidetes (p value 0.01) and Verrucomicrobia (p value 0.01), while pasteurized A. muciniphila showed no significant change in Phylum level (Fig. Akkermansia muciniphila is a commensal bacterium using mucin as its sole carbon and nitrogen source.A. Obes. Honda, K. & Littman, D. R. The microbiota in adaptive immune homeostasis and disease. 7, 4557 (2014). In agreement with our research, the positive role of A. muciniphila in the adipose health and immune status in normal18 and obese mice18,62 were reported. (p value 0.005) abundance in PAm group were observed at the Class/Family level relative to control group (Fig. The expression of tnf- significantly reduced following both treatments, however, there was no significant difference between both of them. Global scientific output trend for Akkermansia muciniphila research: A bibliometric and scientometric analysis. paracasei L. casei W8 on blood levels of triacylglycerol is independent of colonisation. In this study, A. muciniphila dynamic cultures using five different media were implemented in an in vitro bionic intestinal reactor for the first time instead of the traditional static culture using brain heart . Hemarajata, P. & Versalovic, J. Comparative effects of alive and pasteurized, https://doi.org/10.1038/s41598-021-95738-5. We found that both treatments had a beneficial effect on lipid metabolism in the colon of mice, similar to Caco-2. . Immunol. PubMed Akkermansia muciniphila (A. muciniphila), an intestinal symbiont colonizing in the mucosal layer, is considered to be a promising candidate as probiotics.A. 86, e03004-19 (2020). Article Kang, H.-J. Nutr. (A) Histopathology of eWAT of ND-fed mice. B. Adipose tissue remodeling: Its role in energy metabolism and metabolic disorders. It is mandatory to procure user consent prior to running these cookies on your website. Overall, these results suggested that alive and pasteurized A. muciniphila had a direct effect on the gene expression involved in integrity, inflammation, and lipid metabolism. Article The mean relative abundance at (C) Family/Class and (D) Genus. Akkermansia muciniphila is a promising probiotic - PubMed 4C). The down-regulation of tgf- was observed after both treatments (Fig. Akkermansia Muciniphila - Agora Market A healthy gut microbiome is multi-faceted and relies heavily upon all 3 of these factors, like a three-legged stool. J. Nutr. Rev. 5C). 2A). Nataraj, B. H., Ali, S. A., Behare, P. V. & Yadav, H. Postbiotics-parabiotics: The new horizons in microbial biotherapy and functional foods. OMNi-LOGiC PLUS - for a balanced intestinal tract! Scale bar is 50m. A tendency to decrease biochemical parameters level after both treatments were observed, which accompanied by regulating lipid profiles i.e. ppar- and tgf-) in the eWAT of ND-fed mice. Akkermansia muciniphila, a frequent colonizer in the gut mucous layer of individuals, has constantly been recognized as a promising candidate for the next generation of probiotics due to its biological advantages from in vitro and in vivo investigations.This manuscript comprehensively reviewed the features of A. muciniphila in terms of its function in host physiology and frequently . A decrease in Enterobacteriaceae (p value 0.006) and Prevotellaceae (p value 0.008) abundance in Am group as well as in -Proteobacteria (p value 0.02) and Clostridia spp. Moreover, A. muciniphila may have a value in modifying cancer treatment. For the preparation of pasteurized form, the bacteria suspension was heated at 70C for 30min17. The probiotic strain animalis ssp. Positive regulation of the gut microbiota has been proposed as one of the mechanisms underlying probiotic effects64; nevertheless, contradictory data exists regarding probiotics ability to modulate intestinal microbiota in healthy hosts. Phua, T. et al. Note: Take this product with plenty of fluids to ensure that it reaches the stomach; if users have difficulty swallowing or too little liquid is consumed, there is a risk of choking. Moreover, the pasteurized form of the bacterium reduced tlr-4 expression (p value<0.0001) in mice's liver, notably, the live form had more noticeable effect (p value<0.0001). Natl. PubMed Dao, M. C. et al. This study illustrates that consumption of the particular probiotic strain B420 has a novel role in modifying the microbiome in addition to supporting weight management. Changes in gut microbiota control inflammation in obese mice through a mechanism involving GLP-2-driven improvement of gut permeability. One of the next-generation probiotics considered to be a healthy biomarker bacterium in the human and animal intestines is Akkermansia muciniphila (A. muciniphila)10,11. In the present study, the effects of alive and pasteurized A. muciniphila on the integrity of gut barrier, inflammation, and energy homeostasis were assessed in vitro and in vivo. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. 6, 261274 (2011). Nutrients 12, 1474 (2020). Overall, these results indicated that pasteurized A. muciniphila intervention promoted health and prevented the onset of metabolic disorders by lowering effects on lipid profiles, glucose, liver injury-related enzyme, and inflammatory biomarkers in the plasma of study groups. Beneficial Microbes 7, 539547 (2016). Both forms of A. muciniphila decreased TNF-a and IL-6 and also increased regulatory cytokines IL-10 level. Sign up for the Nature Briefing newsletter what matters in science, free to your inbox daily. Once a perfect surrounding has been created, the commensal intestinal bacteria can multiply and perform in the best way possible. Appl. Caco-2 monolayer were inoculated with A. muciniphila (MOI100)and pasteurized A. muciniphila (MOI100) for 24h. The expression of genes: (A) tight junction proteins (zo-1, ocldn, and cldn-1), (B) inflammation-related genes (tlr-2, tlr-4, and tnf-), and (C) angptl4. In addition, reversing effects of alive or pasteurized A. muciniphila on HFD-induced abnormal morphology of tissues revealed in animal models17,18,24,29. Gmeinstrae 13, 8055 Graz Both treatments displayed some changes in Firmicutes/Bacteroidetes ratio; however, there was no statistical difference was observed (Fig. *% NRV= nutrient reference values according to VO (EU) No. Proteins 13, 111 (2021). After acclimation, control and treatment groups were administrated with PBS and 109CFU/200L of bacterial suspension for 5weeks, respectively. and JavaScript. PLoS One 10, e0139795 (2015). One consideration in evaluating the safety of probiotics is the recognition of undesirable changes in immune parameters45, owing to emerging evidence that probiotics or their derivatives may have immunomodulatory impacts. /ID: 74,134), PrimeScript RT Reagent Kit (Takara, Japan, Cat. Dehghanbanadaki, H. et al. 58, 114. https://doi.org/10.1038/s41598-021-95738-5, DOI: https://doi.org/10.1038/s41598-021-95738-5. Hayes, C. L. et al. In addition, the beneficial effects of pasteurized A. muciniphila17 and its extracellular vesicles18,35 have been reported on strengthening intestinal integrity in obese mice, which indicates the positive role of non-viable form of this bacterium. To determine whether para-probiotic and probiotic of A. muciniphila can affect intestinal immune homeostasis in non-inflammatory conditions, we were used mice undergoing standard diet. Environ. As a result, daily supplementation of A. muciniphila generated a reduction in harmful bacteria and an increased population of beneficial microbiota that could be correlated with improving the immune and lipid homeostasis of mice in this study. PubMed Central Indeed, no accumulation of lipids droplets was observed in both treatments, similar to that control groups (Fig. Lactobacillus rhamnosus RC007 intended for feed additive: Immune-stimulatory properties and ameliorating effects on TNBS-induced colitis. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. One of the peripheral organs proved that have a strong association with gut is liver, which is in many respects a reflection of a person's health56 and has multiple functions and cross-talking with other organs. Magne, F. et al. PubMed Derrien, et al. Probiotic B420 and A. muciniphila | Metagenics Institute The aim of this study was evaluation of the effect of alive and . Disruption of intestinal microbiota composition known as dysbiosis has been reported in many diseases and its return to normal pattern has been associated with the amelioration of diseases5, 6. Appl. . 7, 48514860 (2016). Health Med. A. For analysis of the relative difference of gene expression among treatments and control groups in both cell line and mice as well as generation of figures, GraphPad Prism 8.0 (GraphPad Software Inc, CA, USA) was used. Institut AllergoSan https://doi.org/10.1530/JME-16-0054 (2017). *, **, *** 'P<0.05, P<0. Revelo, X. S., Luck, H., Winer, S. & Winer, D. A. Morphological and inflammatory changes in visceral adipose tissue during obesity. In line with these findings, oral daily A. muciniphila administration improved liver health by reducing lipid accumulation, downregulating FA oxidation-related genes, and reducing chronic low-grade inflammation in normal mice7. However, most of the current researches focus on the . zo-1, ocldn, and cldn-1 and also decreasing cldn-2 mRNA level. and A. muciniphila. analyzed data. Google Scholar. We also use third-party cookies that help us analyze and understand how you use this website. carried out animal and other experiments. 19, 122 (2020). Glucose Metabolism and Akkermansia. It has been also reported that the live multi-strain probiotics were more effective than the non-viable probiotic strains in in improving insulin resistance associated with intestinal microbiota alteration, butyrate production, and il-10 induction48. Expression of (B) tight junction proteins (zo-1, ocldn, cldn-1, and cldn-2), (C) inflammation-related genes (tlr-2, tlr-4, tnf-, and il-10), and (D) angptl4. Immunol. 3A). PubMed Central On the other hand, a high abundance of A. muciniphila is linked to decreased blood lipids and improved metabolic features that ultimately lead to a healthy state in obese adults15. Commensal microbiota induces colonic barrier structure and functions that contribute to homeostasis. In addition, when overweight or obese people undergo calorie-restricted diet therapy, the effect of improving insulin resistance has . 8, 266 (2017). 19, 219232 (2018). Genes Nutr. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. PubMed Med. Notably, alive A. muciniphila had better immunomodulatory and lipid-regulating effects on the liver of mice. Jacouton, E. et al. Valentini, M. et al. This two-way communication is accomplished through various mechanisms, including TLRs4. Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Today, in addition to common probiotics, next-generation probiotics, such as Akkermansia muciniphila (A. muciniphila) and Faecalibacterium prausnitzii (F. prausnitzii), have been proposed. PubMedGoogle Scholar. Thus, improved metabolic vital tissues structure in A. muciniphila-fed mice can be due to positive effects of the probiotic and para-probiotic on digestion, absorption, nutrient usage, and metabolism. Moreover, A. muciniphila may have a value in modifying cancer treatment. J. Clin. Adv. Overall, results showed that alive A. muciniphila treatment can maintain healthy gut microbiota pattern and promote health. Cani, P. D. et al. Akkermansia muciniphila, which is enriched in the gut microbiota by Real-time PCR was performed using SYBR Premix Ex Taq II (Takara). Scale bar is 50m. Depommier, C. et al. MegaSporeBiotic is the first 100% spore-based, broad-spectrum probiotic clinically shown to improve leaky gut by 60% in just 30 days. Authors Our data demonstrated that alive and pasteurized A. muciniphila had a substantial link with intestinal and immune homeostasis, which highlighted their key role in health promotion. Angiopoietin-like 4 mediates colonic inflammation by regulating chemokine transcript stability via tristetraprolin. Ginkgo Ginseng Glucosamine Herbal Homoeopathy Iron Multivitamin Omega 3 Fatty Acids Probiotics Turmeric & Honey Vitamin B12 Vitamin C Vitamin D Zinc Detox Seasonal Allergy Prescription only Bone Health Brain Sexual Health Skin Care Cardiovascular Health Slimming Circulation Sports Nutrition A significant alleviation in gene expression of tlr-4 and significant upregultion in tlr-2 were observed in the colon of pasteurized A. muciniphila-gavaged mice, compared to control group (p value 0.02 and<0.0001, respectively). Allam-Ndoul, B. et al. Caco-2 cell line was used to investigate direct effects of live and pasteurized A. muciniphila on the intestinal barrier function and inflammation in intestinal epithelial cells. J. Mol. Exp. Therefore, both forms of A. muciniphila could modulate lipid and immune homeostasis, improved some gut microbiota, and promoted the overall health, while all these effects were dominantly observed in pasteurized form. Assists weight loss as part of a calorie-controlled diet (Glucomannan).