Application Area: Anisotropy and bacteria growth, Organization: Aragen Life Sciences pvt ltd, Application Area:Fluorescence polarization, Kinetics. PDF COMPACT MANUAL USE OF SPARK M10 PLATE READER - Radboud Universiteit By continuing to browse our website, you accept our cookie policy. The machine runs very quickly and efficiently. Spark - Tecan The Spark 10M Multimode Microplate Reader has added fast throughput capabilities to our spectroscopy laboratory. Spark instrument specifications and typical performance values document.write(new Date().getFullYear());, Tecan Trading AG, Switzerland, all rights reserved. Use Tecan Spark multimode microplate reader to measure absorbance or fluorescence intensity of green fluorescence protein. One of the disadvantages of the software used in Tecan Spark 20M, is that it doesn't give you a calculation of the time-to-end of your assay screening after you've programmed the detection actions to be done. HMIL has developed techniques to culture strict anaerobic gut bacteria at scale and built an extensive culture collection for phenotypic profiling. One-click applications streamline your most common assays, getting you from sample to results faster than ever before. The innovative design of Sparks High Speed Monochromator delivers an absorbance spectrum between 200-1,000 nm in just five seconds using a step size of just 1 nm, effectively eliminating the risk of sample evaporation. If yes please follow these steps: 1) Select the correct plate and the wells that you want to measure 2) Add in your protocol in Method. Sophisticated and high quality instrument. Prt pour ceux de demain. Das Spark Motion-Konzept von Tecan ermglicht die Automation von zellbasierten Assays mit bis zu 40 Platten ber mehrere Tage ohne jegliche Benutzerinteraktion. Unmatched flexibility for any assay or budget. The Spark offers a number of features to meet these demands and to ensure optimal assay performance, including Tecans unique Fusion Optics which combine the sensitivity of filters and the flexibility of monochromators (MCRs) in one instrument. The ingenious Fusion Optics in the Spark multimode reader, Evaluation of the performance of the Spark multimode reader for far red fluorescence emission, Homogeneous time-resolved fluorescence (HTRF) assay technology in the Spark multimode reader, Unparalleled flexibility for luminescence detection with the Spark multimode reader, Implementation of AlphaLISA technology in the Spark, Implementation of AlphaScreen technology in the Spark multimode reader, Automated monitoring of metabolic activity and differentiation of human mesenchymal stem cells, Real-time analysis of stem cell proliferation during differentiation, Walk-Away Monitoring of Cytotoxicity, Viability and Apoptosis, Fluorescence top & bottom - incl. Mikroplatten-Reader mit Lebendzell-Bildgebung und Echtzeit - Tecan Each quartz micro-spot is shaped to function as an individual lens guaranteeing the best possible light transmission through the sample and can be quickly and easily cleaned to minimize the risk of cross-contamination. This technical note demonstrates the Spark readers suitability for the generation of growth curves in the commonly used 24- and 96-well plate formats for three different, well-established bacterial strains, Escherichia coli, Listeria innocua and Staphylococcus capitis. Very easy to use. Ce dispositif associe une imagerie sophistique des capacits de lecteur multimode prouves, ce qui vous permet de dfinir de nouvelles approches pour votre recherche et d'obtenir des donnes solides plus vite que jamais auparavant. View Pre-analytical automation: An evaluation of the TECAN genesis FE-500 I got a couple of quite for different machines and the Tecan seemed best value for money. Latest: Environmental Analysis, Pesticides, Contaminants & Sustainability: Technologies and Latest: 6 upcoming webinars to boost your research, Latest: Materials science: Key technological advancements to celebrate. Dr Gediminas Vidugiris of Promega and Dr Katrin Flatscher of Tecan talk about the temperature susceptibility of many biochemical assays and how temperature fluctuations in the lab can distort measurement results. This unique feature is available for all fluorescence-based detection modes including standard top and bottom measurements, TRF, FRET, TR-FRET and FP delivering very low detection limits and unlocking new possibilities. Overall, great product from a great company. The SPARK instrument has been the best reader for analyzing our samples, proceeded from being candidates for new drugs. I am able to perform kinetics analysis and with the temperature/CO2 control and humidity cassette, I can do long kinetics over the day. This system allows researchers to freely configure a reader to their needs and full upgradeability allows access to all techniques and features in the future. Reproducible results obtained. This feature allows you to pause and resume kinetic experiments, enabling additional measurements to be performed throughout long-term studies. of luminescent proteins) can be easily set - using an innovative filter-based approach, users can select any wavelength between 398 nm and 653 nm, with a step size of 15 nm and a 25 nm bandwidth. We already had a similar product, so nothing much to add. This allows you to achieve very low CVs with high intra- and inter-well reproducibility when measuring adherent cells in microplates. Simply pipette 2 l samples from your fluorescence assay for example, the Quant-iT PicoGreen dsDNA assay onto the NanoQuant Plate and use Spark to detect the fluorescence signal. Safeguard your kinetic assays using automated gain regulation to avoid fluorescence measurements running into saturation. The innovative design of the luminescence multicolor optics not only enables for spectral recording of luminescence signals without losing instrument sensitivity. The system now after update of the software became an essential tool in the lab! Spark-Reader und Software von Tecan verbessern die Durchfhrung von Fluoreszenzversuchen in Mikrotiterplatten. document.write(new Date().getFullYear());, Tecan Trading AG, Switzerland, all rights reserved. Upgradeable absorbance plate reader delivering sensitive results for ELISAs and low volume nucleic acid or protein quantification assays. The new cell counting function has proven rapid, precise and very helpful in my laboratory and has fully replaced the conventional counter. Best product - highly flexible to use for different application with reproducible results. Our Spark is equipped with a monochromator for absorbance and filters for fluorescence assays. Automated Z-position optimization and an integrated dichroic mirror that is perfectly suited to HTRF measurements further increase the Sparks outstanding sensitivity. Reliable results are dependent on a stable temperature. The software allows you to easily translate your protocol into a method that can be run or edited at any time. There is therefore a need for accurate methods to study these pathogens, and a common way to do that is through turbidimetric measurements. It is useful for a broad range of applications, such as DNA or RNA quantification, nucleic acid purity assessments and determination of labeling efficiency. SparkControl software provides effortless control of the system, enabling easy selection of filters or monochromators on the fly. Sparks unique ability to combine the flexibility of monochromators with the sensitivity of filters is especially relevant for fluorescence emission or excitation scans. The method editor is very convenient and easy to use for creating new protocols. Imagine the time delays and decrease in productivity you could avoid in a multi-user lab by just shortening that cool-down window. Developed with cell-based assays in mind, it offers a host of software and hardware features, including a unique Te-Cool cooling module that delivers total temperature control, even below ambient. This application note shows that the Optimal Read function produces the most reliable results for the analysis of adherent cell types, giving the highest well-to-well uniformity and best sensitivity for cell-based applications. Our instrument actually had to be serviced right away because the issue with the cell counting module was detected during set up. Another limitation is that the reader cannot do a BRET assay. The Tecan Spark M10 multimode plate reader has the following modules: Multiple types of plate and wells Absorbance reading with monochromator optics (200-1000nm) - Fluorescence top / bottom reading with monochromator for Exc (230-900nm) and Em (280-900nm), also step-wise intensity scans over range Fluorescence polarization reading >390nm The instrument can also handle any model of microplates. Simply add ddH2O to the filling moat and you have an instant, no-fuss humidified atmosphere in your microplate chamber. ZERO BIAS - scores, article reviews, protocol conditions and more Designed to improve sensitivity and increase throughput, this set-up allows on-the-fly measurements, increasing productivity without compromising on data quality. Tecans Spark multimode reader has an integrated, bright field cell imaging module. It is also the capital city of the province of Flevoland. No matter what your application, the Spark 10M multimode microplate reader delivers the ideal combination of advanced capabilities and exceptional ease-of-use, enhancing productivity with a series of unique features and benefits. Monitoring growth of prokaryotic cells and yeast is an important tool in life sciences, as well as in the food industry. Spark Multimode Microplate Reader by Tecan Manufacturer Tecan | Available Worldwide 4.5 / 5.0 | 49 reviews | Write your own review Increase your productivity for advanced research and drug discovery with Spark, the first multimode microplate reader with integrated cooling. *************************************************************************************** Received a comprehensive training on the magellan software! Smart automation is the harmonious combination of Sparks revolutionary technologies with your research, unlocking new possibilities. As we have seen in the previous posts in this series, implementing fluorescence detection will be a quick and effective route to improving the quality and sensitivity of your assays. Especially the setup of new measurement technologies is very fast done as it is very intuitive. Even resolution of small cell clusters does not pose a problem. Application Area:Quantitative analysis of biomarkers, Organization: University Hospital Dusseldorf, Germany, Application Area:Measuring absorbance in EIA determinations, Organization: University of North Texas HSC, Application Area:protein estimation, quantification of fluorescent & luminiscence intensity of the cells, Organization: University of California San Diego, Application Area:Analyze luminescence and fluorescence, Application Area:protein binding and cell absorbance assay, Organization: California Institute of Technology, Application Area:Luminescence, fluorescence, DNA quantification, Application Area:protein and DNA quantification assays. For most of the time i load protein in SDS page gel depending upon the estimation by BCA, assessed by Tecan Spark and i always gets almost same intensity of control band. *************************************************************************************** A lens-based bottom reading system, combined with the Z-focusing guides the light to a focal point on the sample that delivers outstanding sensitivity. There are so many options available when ordering the instrument that it can easily fulfil everyone's needs. With the live viewer in SparkControl, you can document the last view before starting the experiment with the Snapshot function. The surface to set up a method is easily understood and very flexible in terms of adding or reducing the number of samples or blocks to be used in each method. . It typically takes over 60 minutes for a reader to cool down from 37 C to room temperature through passive cooling, costing you valuable time and disrupting your busy schedule. Cons: The Firmware updates must be done regularly. Spark adds another layer of environmental controls by putting CO2/O2 partial pressure control at your fingertips. This system offers an affordable solution for users wanting to enter this exciting application area. Lecteur de plaques absorbance, fluorescence, et luminescence - Tecan Which smart sample prep solution best fits your application. The Spark multimode microplate reader offers walkaway: Please note that Spark is for research use only. Spark is equipped with precise temperature control to enable uniform heating of your microplate. If there's a will there's a way, the capabilities seem endless! For most readers, the specified minimum temperature inside the measurement chamber is defined as a few degrees above ambient. SparkControls kinetic conditioning feature allows you to program the system to automatically perform a required action as soon as a predefined signal is reached in a control well, fitting almost any assay workflow you can think of, in un-lidded or lidded microplates. They discuss ways to achieve stable temperature conditions throughout the entire experimental workflow using the Te-coolTM active cooling functionality in the Spark microplate reader. *************************************************************************************** Combining the NanoQuant Plate and Spark offers the ultimate solution for nucleic acid quantification. It is a homogenous, competitive assay based on the detection of ADP with an extremely sensitive antibody against ADP. Spark Troubleshooting Magellan How To Luminescent signal over time in a kinetic measurement. Spark uses luminescence fibers optimized for each plate format 96, 384 and 1,536 wells guaranteeing minimum signal crosstalk between wells. Tecans Spark multimode microplate reader was used to identify metabolic changes during the differentiation of human mesenchymal stem cells and to assess and quantify cell viability, cytotoxicity and apoptosis, using several well-established and reliable cell-based assays developed by PromoCell. Here's what I usually do with it: A patented Humidity Cassette reduces evaporation in standard microplates, enabling long-term live kinetic studies without the need to switch to dedicated and costly microplate types simply use your current, validated plates. Highly reproducible results with complete Tecan-based drug screening pipeline. Each aspect is meticulously manufactured to ensure that the data you produce is true and consistent, empowering your discovery. Affordable imaging for increased data confidence. He studied Biology at Gutenberg University Mainz and Trinity College Dublin and received a PhD in Biochemistry from Goethe University Frankfurt am Main. SparkControls one-click applications epitomize our effort to put fast, simple instrument operation at your fingertips. The light is guided through an innovative filter wheel consisting of multiple attenuation filters reducing high signals by a factor of up to 1,000 and 38 user-selectable colored filters, enabling virtually any multi-color luminescence measurement. Sparks Humidity Cassette is a cost-effective solution to minimize evaporation in any SBS-format microplate. Analytical Core | California NanoSystems Institute | California Right now we are establishing two methods for two different tests which will be very useful for us in the lab to gain a deeper insight into our cell culture Performance. Open Kinetic allows you to pause and resume kinetic reads. But they have been in touch to check everything is working. Tecan uses cookies to improve our website. Schnelle Absorptionsmessung in Mikrotiterplatten und Kvetten mit Monochromator. Application Area:Monitor growth curves, fluorescent probe uptake into whole cells. Latest: Celebrating the scientists and technologies changing the world: Dont miss our 25th Latest: Volumetric dried blood sampling in a patient and lab friendly device: A game-changer for Latest: The top 10 products accelerating pharmacology, Latest: Svar Life Science announces the launch of GMP QC testing services. HTRF assays place high sensitivity demands on multimode readers. This allows incubation and monitoring of cell culture microplates within the measurement chamber, enabling the user to define the confluence at which the assay starts/ends or the substrate is injected, for optimal assay results and greater walkaway operation. The high-power xenon flash lamp reduces the number of flashes needed for reliable and sensitive results, so you dont have to compromise between sensitivity and speed. This application note describes the implementation of NanoBRET technology on the Spark multimode reader, using the NanoBRET Target Engagement Intracellular HDAC Assay. One last look. It's a multimode microplate reader which means you can do various experiments with it. Spark Cyto is a multi-mode plate reader with fluorescence imaging and cytometry capabilities, unlocking new possibilities for your cell-based research. Sparks fluorescence module is available with a choice of filters, monochromators or Fusion Optics a novel combination of both monochromators and filters. A good wavelength ratio accuracy in A260/280 or A260/230 is crucial for DNA/RNA purity checks, ensuring repeatable and reproducible data, but this parameter is not specified for most systems on the market. Unique heating and stirring capabilities reduce the risk of precipitation. Bioz Stars score: 86/100, based on 1 PubMed citations. Measurements with different gain settings are then automatically correlated, allowing evaluation of the entire dataset. The ability to generate reliable, reproducible and high quality data should be a prerequisite for all laboratory instruments. Tab 04 / Accessories Instrument is upgradeable post-purchase, so additional functionality can be added as needed. After-sales care is excellent!! The Humidity Cassette can minimize the impact of edge effects in microplates under certain conditions. The latest-generation BRET assay, NanoBRET, employs an extremely bright NanoLuc luciferase and a long-wavelength acceptor fluorophore, resulting in a markedly improved detection sensitivity and a higher dynamic range. The Transcreener ADP2 TR-FRET Red Assay is a competitive immunoassay for ADP with a far-red, time-resolved fluorescence-resonance-energy-transfer (TR-FRET) read-out. Dr Katrin Flatscher, Application Scientist, and Dr Michael Fejtl, Market Manager Detection, at Tecan, will describe application of the SparkMultimode Reader imaging capabilities for monitoring cell migration in wound healing, using the Radius 96-well Cell Migration Assay as a test system. Le lecteur Spark vous permet de combiner votre gr une grande slection de modules et vous donne ainsi toute la flexibilit ncessaire pour configurer un systme qui correspond exactement vos besoins. It describes the established methods to generate OD 600 growth curves of all three bacterial strains in 24- and 96-well plates over a period of 8 hours. Tecan offers microplate readers with both multimode or absorbance reading options. But isnt your data too important to compromise? The design of taking out the injector out of the unit to prime and put it back and forth for injection is kind of difficult especially when injector is used a lot. ***************************************************************************************. SparkControl Magellan is a data analysis package that provides you with powerful data reduction tools for numerous detection modes. This product is extremely easy to use and reads plates fast. This is complemented by Sparks Te-Cool module for stable temperature management, even below ambient, giving you full control over this critical assay parameter. Sparks innovative Te-Cool cooling module provides complete temperature control between 18 and 42 C allowing you to maintain your specified temperature for long periods of time, for accurate and reliable results.